2 research outputs found
Enhanced Extracellular Polysaccharide Production and Self-Sustainable Electricity Generation for PAMFCs by <i>Scenedesmus</i> sp. SB1
In this study, a freshwater microalga, <i>Scenedesmus</i> sp. SB1, was isolated, purified, and identified
by its internal
transcribed spacer region (ITS1-5.8S-ITS2). Media optimization through
the Plackett–Burman Design and response surface methodology
(RSM) showed a maximum exopolysaccharide (EPS) production of 48 mg/L
(1.8-fold higher than that for unoptimized media). Characterization
using gas chromatography–mass spectrometry, Fourier transform
infrared, X-ray diffraction, and thermogravimetric analysis reveals
that the EPS is a sulfated pectin polysaccharide with a crystallinity
index of 15.2% and prompt thermal stability. Furthermore, the photoelectrogenic
activity of <i>Scenedesmus</i> sp. SB1 inoculated in BG-11
and RSM-optimized BG-11 (ROBG-11) media was tested by cyclic voltammogram
studies, revealing the potential of the inoculated strain in ROBG-11
toward photosynthetic algal microbial fuel cells over normal BG-11.
To the best of our knowledge, functional group characterization, physical
and thermal property and media optimization for EPS production by
RSM and electrogenic activity studies are reported for the first time
in <i>Scenedesmus</i> sp. SB1